Re-engineering multicloning sites for function and convenience

Re-engineering multicloning sites for function and convenience

Multicloning sites (MCSs) in standard expression vectors are widely used and thought to be benign, non-interacting elements that exist for mere convenience. However, MCSs impose a necessary distance between promoter elements and genes of interest. As a result, the choice of cloning site defines the genetic context and may introduce significant mRNA secondary structure in the 5'-untranslated reg...

compactifications and function spaces on weighted semigruops

chapter one is devoted to a moderate discussion on preliminaries, according to our requirements. chapter two which is based on our work in (24) is devoted introducting weighted semigroups (s, w), and studying some famous function spaces on them, especially the relations between go (s, w) and other function speces are invesigated. in fact this chapter is a complement to (32). one of the main fea...

Re-engineering is dead; Long live re-engineering

Business process re-engineering (BPR) has been associated with runaway costs and corporate downsizing. This and other negative connotations have created or strengthened suspicions about the organizational impacts of information technology (IT). This paper considers the fundamental ideas of BPR, with IT as an enabling agent, and examines their consistency with sound management practice. © 1997 E...

Membrane Engineering for Today and for Tomorrow

Grammar Re-Engineering for Language Centered Software Engineering

This paper describes a systematic approach to grammar re-engineering by transforming conventional restricted YACC grammars from legacy code into the purely declarative syntax definition formalism SDF. The declarative nature of SDF and its ability to handle the full class of context-free grammars allows grammar definitions that are more readable and maintainable compared to the original YACC gra...